Amniotic Membrane

Amniotic membrane is used by Dr. Duplessie to protect, repair and heal damaged eye surfaces

Amniotic membrane works by providing and underlying structure called basement membrane which permits normal skin or conjunctiva to grow on it. It does this by reducing inflammation and scarring.  It also reduces the formation of new blood vessels which cause only problems in the cornea.

 

Amniotic Membrane Transplantation Limitations:

The following may complicate the survival of your amniotic membrane transplant

  • Extreme dry eye
  • Severe lack of blood flow
  • Severe lack of stem cells

Clinical Uses of Amniotic Membrane Transplantation


Amniotic Membrane Used as a Temporary Graft

 

Major Action of Amniotic stem cell transplant:

Can help suppress undesirable inflammation but severe inflammation may require the use of multiple sequential amniotic grafts.  The end result is for surface healing without pain or scarring.

Indications for amniotic transplantation:
  • Acute chemical burns
  • Acute Stevens Johnson syndrome
  • High-risk Corneal Transplants
  • Severe keratitis or limbitis with or without stromal ulceration –HSV, HZO, Vernal, Phlyctenular
  • Post Eximer laser ablation in Phototherapeutic keratectomy



Amniotic Membrane Used as a Permanent Graft for Corneal Surface Reconstruction

 

Major Action of Amniotic Stem cell transplant:

Restores corneal integrity by replacing the basement membrane which contributes to corneal skin growth and reduces inflammation and scarring during healing.


Skin missing in center of cornea

Blister [bullae] on cornea

  • Band keratopathy
  • Persistent corneal epithelial defect, ulcer, decemetocele, orperforation
  • Non-healing neurotrophic ulcers

 

Amniotic Membrane Used a Permanent Graft for Conjunctival Surface Reconstruction

 

Major Action:

To restore the conjunctival after severe damage from disease, infection, inflammation or surgery.

 

Indications for Amniotic transplant of Conjunctiva:

  • Benign (pinguecula) or dysplastic or malignant tumors
  • Pterygium (primary or recurrent, single-headed or double-headed)
  • Symblepharon
  • Conjunctivochalasis
  • Superior limbic keratoconjunctivitis
  • Leaking, cystic or infected blebs
  • Obliterated fornix (contracted sockets)
  • With or without scleral melt
  • With or without implant extrusion



Amniotic Membrane Used for Reinforcing Tenon’s Capsule


Major Action:

To restore the subconjunctival Tenon’s capsule been weakened or damaged by the disease

Indications:
  • Conjunctivochalasis
  • Superior limbic keratoconjunctivitis
  • Fat prolapse



Amniotic Membrane Transplantation with Other Procedures

  • With fibrin glue most commonly in Pterygium surgery.  Helps prevent recurrence.
  • With Intraoperative Application of Mitomycin C: Helps prevent recurrence
  • With Tenonplasty- To reduce stromal ischemia and necrosis
  • With Other Grafts- To reduce the size of the graft otherwise needed